Journal: STAR Protocols
Article Title: Protocol for investigating tertiary lymphoid structures in human and murine fixed tissue sections using Opal™-TSA multiplex immunohistochemistry
doi: 10.1016/j.xpro.2022.101961
Figure Lengend Snippet: Proposed grading system for the accurate classification of immune cell infiltrates, immune cell aggregates, immature TLSs and mature TLSs using the 7-marker TLS panel on human prostate cancer FFPE tissue sections Representative ROIs showing merge staining: CD4 (red), CD8 (magenta), CD19 (green), CD21 (yellow), DC-LAMP (orange), PNAd (cyan), and DAPI (blue). Images were taken at 20× magnification, and scale bars indicate 100 μm.
Article Snippet: Rabbit polyclonal anti-mouse CD19 antibody , Cell Signaling Technology , Cat#3574 (1:200 dilution).
Techniques: Marker, Staining